|
KMID : 0379120030310020089
|
|
Korean Journal of Mycology
2003 Volume.31 No. 2 p.89 ~ p.93
|
|
|
Comparison of Growth Characteristics of Tricholoma matsutake Mycelium Among the Types of Air Bubble Bioreactor
|
|
Lee Wi-Young
Ka Kang-Hyeon
Ahn Jin-Kwon
Kwan Young-Jin
|
|
|
Abstract
|
|
|
|
In order to select suitable bioreactor type inhibiting cell stress during submerged culture of Tricholoma matsutake mycelium, the growth characteristics and ergosterol contents were investigated using the external-loop type of air-lift bioreactor (ETAB), balloon type of air bubble bioreactor (BTBB) and column type of air bubble bioreactor (CTBB). Dry weights of the T. matsutake in the BTBB, ETAB and CTBB were 12 g, 11.4 g, and 9.5 g per 1 litter, respectively. BTBB, ETAB and CTBB reached stagnant phases 16, 20, and 24 days after cultivation, respectively, The BTBB was more suitable for liquid culture of T. matsutake mycelium compared to other bioreactors owing to much mycelia product and short culture period. The ergosterol contents produced by the mycelium in the bioreactors were in sequence of BTBB, CTBB, and ETAB at every growth phase. BTBB might affect the mycelium on producing the smallest size of pellets. BTBB and CTBB got the mycelium precipitated and coagulated under operation of bioreactor sparser, whereas ETAB shown no effect of above phenomenon. A renovated bioreactor combined between a balloon shape of BTBB and an external-loop of ETAB was developed to enhance the efficiency of culture technique.
|
|
|
KEYWORD
|
|
|
Air bubble bioreactor, Balloon type air bubble bioreactor, Ergosterol, Mycelial culture, Tricholoma matsutake
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|